Multiplex real-time PCR for detection of respiratory tract infections
نویسندگان
چکیده
منابع مشابه
multiplex sybr green real-time pcr assay for detection of respiratory viruses
conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (11.04%) piv3, 9 (5.23%) piv4, and 1 (0.58%) coronavirus nl63. all the positive samples were detected during december t...
متن کاملMultiplex PCR theranostics of severe respiratory infections.
Scope of the problem Acute respiratory tract infection is the leading cause of hospitalization for infants and young children in developed countries, and a major determinant of death in developing countries. In 2002, lower respiratory tract infections accounted for approximately 4 million deaths worldwide and 7% of all deaths that year [1]. Therefore, there is a huge need for new diagnostic too...
متن کاملMultiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses
BACKGROUND It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The development of molecular methods for direct detection of viruses has been progressed recently. OBJECTIVES The objective ...
متن کاملMultiplex real-time PCR assay for detection of influenza and human respiratory syncytial viruses.
A multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus (HRSV). Detection of each viral product and of an internal control was based on determination of specific melting temperatures by the LightCycler software. The lower limit of detection in the multiplex PCR assay was found to be 50 copie...
متن کاملReal-time multiplex PCR assays.
The ability to multiplex PCR by probe color and melting temperature (T(m)) greatly expands the power of real-time analysis. Simple hybridization probes with only a single fluorescent dye can be used for quantification and allele typing. Different probes are labeled with dyes that have unique emission spectra. Spectral data are collected with discrete optics or dispersed onto an array for detect...
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ژورنال
عنوان ژورنال: Journal of Clinical Virology
سال: 2008
ISSN: 1386-6532
DOI: 10.1016/j.jcv.2007.10.029